Lyophilization of cell-free Marek's disease herpesvirus and a herpesvirus from turkeys.
نویسندگان
چکیده
Cell extracts of the JM and GA strains of Marek's disease herpesvirus and the FC 126 strain of turkey herpesvirus were lyophilized with various stabilizers. Much higher virus titers were obtained with stabilizer than without stabilizer. Titers increased even further in the case of the Marek's disease virus strains by the addition of a chelating agent, disodium ethylenediaminetetraacetate.
منابع مشابه
Identification with monoclonal antibodies of glycoproteins of Marek's disease virus and herpesvirus of turkeys related to virus neutralization.
By use of monoclonal antibodies cross-reactive with Marek's disease virus and herpesvirus of turkeys, three glycoproteins (for Marek's disease virus, gp115/110, gp63, and gp50; for herpesvirus of turkeys, gp115, gp62 and gp52) related to virus neutralization were identified. Immunization of chickens or rabbits with these glycoproteins purified by affinity chromatography resulted in production o...
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Phosphonoformate was found to be an inhibitor of the deoxyribonucleic acid polymerase induced by the herpesvirus of turkeys. The apparent inhibition constants were 1 to 3 muM. Phosphonoformate was also able to block the replication in cell culture of Marek's disease herpesvirus, the herpesvirus of turkeys, and herpes simplex virus. It was as effective as phosphonoacetate. Phosphonoformate was n...
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Leukocytes recovered from birds inoculated with an oncogenic strain of Marek's disease virus synthesized deoxyribonucleic acid in vitro at a rate 7.9 times that of leukocytes from normal birds or birds inoculated with the nononcogenic herpesvirus of turkeys. A close relationship was observed between the level of in vitro deoxyribonucleic acid synthesis and in vivo tumor formation.
متن کاملMost virus-specific polypeptides in cells productively infected with Marek's disease virus or herpesvirus of turkeys possess cross-reactive determinants.
Most virus-specific polypeptides in cells productively infected with Marek's disease virus (MDV) or herpesvirus of turkeys (HVT) possess cross-reactive antigenic determinants, although the two viruses have very little DNA homology. The cross-reactivity appeared to be more evident when [35S]methionine-labelled polypeptides were immunoprecipitated than when the [3H]glucosamine-labelled polypeptid...
متن کاملSerological relationships among herpesviruses: cross-reaction between marek's disease virus and pseudorabies virus as detected by immunogluorescence.
Marek's disease virus (MDV) and pseudorabies virus (PRV) cross-reacted by fluorescent antibody test but not by gel diffusion, virus neutralization, or cross-protection tests. Herpesvirus of turkeys cross-reacted with MDV but not with PRV. Other herpesviruses tested did not have cross-reactions.
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ورودعنوان ژورنال:
- Applied microbiology
دوره 20 5 شماره
صفحات -
تاریخ انتشار 1970